Oral Fluids Testing

Oral Fluids testing offers an easy and less obtrusive collection method of biological specimens for drug screening. It utilizes a competitive micro-plate immunoassay for the detection of a drug in an oral fluid collected with an oral specimen collection device. Testing sample, a control, or a calibrator is added to an EIA well along with an enzyme-labeled hapten derivative. A positive result indicates that there is competition between the drug and the enzyme-labeled hapten to bind to the antibody coated well. EIA wells are washed, and then substrate is added which produces a blue color. There is an inverse relationship between the absorbance of each well which is measured at 450nm and the amount of the drug present in the testing sample or calibrator/control.

Drugs commonly detected with this method include:

• Amphetamine
• Barbiturates
• Benzodiazepines
• Cannabinoids
• Cocaine
• Methadone
• Methamphetamine
• Opiates
• PCP
• Belladonna Alkaloids (Jimson Weed)